Journal: Scientific Reports

Article Title: Correlations between TBL1XR1 and recurrence of colorectal cancer

doi: 10.1038/srep44275

Figure Lengend Snippet: ( A ) The expression level of TBL1XR1 in SW480 cells was higher than that in NCM460 cells (normal epithelial cell line), which was consistent with the RT-qPCR and IHC results. ( B ) Overexpression of TBL1XR1 in SW480 cells can increase the transcription activity of β-catenin, while TBL1XR1 knock-down showed opposite effect. ( C ) TBL1XR1 overexpression can up-regulate the protein level of Vimentin, Snail, TWIST1, and N-cadherin, while the E-cadherin expression was down-regulated. On the other hand, competitive inhibition of β-catenin can impair the EMT protein expression. MTT assay ( D ) and Transwell assay ( E ) showed that TBL1XR1 can regulate the proliferation and invasion capacities of SW480 cells through Wnt-β-catenin signaling pathway.

Article Snippet: Blots were blocked for 1 h in 5% BSA and then incubated overnight with the primary antibodies: TBL1XR1 (HPA019182, Sigma), Vimentin (10366-1-AP, Proteintech), Snai1 (13099-1-AP, Proteintech), Slug (12129-1-AP, Proteintech), TWIST1 (18125-1-AP, Proteintech), N-cadherin (13769-1-AP, Proteintech), E-cadherin (20874-1-AP, Proteintech), Cyclin D1 (60186-1-Ig, Proteintech), c-Myc (10828-1-AP, Proteintech) and β-actin (sc-1616, Santa Cruz).

Techniques: Expressing, Quantitative RT-PCR, Over Expression, Activity Assay, Knockdown, Inhibition, MTT Assay, Transwell Assay

Journal: Cells

Article Title: Impact of RBMS 3 Progression on Expression of EMT Markers

doi: 10.3390/cells13181548

Figure Lengend Snippet: Immunohistochemical reactions performed on invasive ductal breast carcinoma tissue revealed expression of RBMS 3 and EMT markers ( A ) N-Cadherin, ( B ) RBMS 3, ( C ) E-Cadherin, ( D ) SLUG, ( E ) SNAIL, ( F ) TWIST 1, and ( G ) representation of negative ZEB 1 staining and positive in stroma of IDC. Magnification ×400. Created with BioRender.com.

Article Snippet: Immunohistochemical reactions were carried out using the following antibodies: RBMS 3 (1:200, PA5-57028, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TWIST 1 (1:50, ab50581, Abcam, Cambridge, UK), SNAIL (1:200, 13099-1-AP, ProteinTech, Rosemont, IL, USA), E-Cadherin (RTU, IR059, Dako, Agilent Technologies, Santa Clara, CA, USA), N-Cadherin (1:50, M3613, Dako, Agilent Technologies), SLUG (1:50, sc-166476, Santa Cruz, Dallas, TX, USA) and ZEB 1 (1:100, ab203829, Abcam).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Scientific Reports

Article Title: Correlations between TBL1XR1 and recurrence of colorectal cancer

doi: 10.1038/srep44275

Figure Lengend Snippet: ( A ) The expression level of TBL1XR1 in SW480 cells was higher than that in NCM460 cells (normal epithelial cell line), which was consistent with the RT-qPCR and IHC results. ( B ) Overexpression of TBL1XR1 in SW480 cells can increase the transcription activity of β-catenin, while TBL1XR1 knock-down showed opposite effect. ( C ) TBL1XR1 overexpression can up-regulate the protein level of Vimentin, Snail, TWIST1, and N-cadherin, while the E-cadherin expression was down-regulated. On the other hand, competitive inhibition of β-catenin can impair the EMT protein expression. MTT assay ( D ) and Transwell assay ( E ) showed that TBL1XR1 can regulate the proliferation and invasion capacities of SW480 cells through Wnt-β-catenin signaling pathway.

Article Snippet: Blots were blocked for 1 h in 5% BSA and then incubated overnight with the primary antibodies: TBL1XR1 (HPA019182, Sigma), Vimentin (10366-1-AP, Proteintech), Snai1 (13099-1-AP, Proteintech), Slug (12129-1-AP, Proteintech), TWIST1 (18125-1-AP, Proteintech), N-cadherin (13769-1-AP, Proteintech), E-cadherin (20874-1-AP, Proteintech), Cyclin D1 (60186-1-Ig, Proteintech), c-Myc (10828-1-AP, Proteintech) and β-actin (sc-1616, Santa Cruz).

Techniques: Expressing, Quantitative RT-PCR, Over Expression, Activity Assay, Knockdown, Inhibition, MTT Assay, Transwell Assay

Journal: Nature Communications

Article Title: Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice

doi: 10.1038/s41467-021-22822-9

Figure Lengend Snippet: a Gene expression heatmap of spiny mouse epidermis at different post-wound days. b Proliferation of epithelial cells and cell movement associated genes in the spiny mouse wound epidermis. c Twist1 related transcription factors in the spiny mouse wound dermis. d ECM remodeling in the spiny mouse wound dermis. e IHC of Twist1, Zeb2, MMP9, E-cad and P-cad in PWD14 the regenerating hair placodes of the spiny mice. White arrows point to the downregulation of E-cad at the tip of hair placode. f The effects of Twist1 inhibitor Harmine and pan-MMP inhibitor GM6001 treatment on hair neogenesis in spiny mouse. Red: wound border; yellow arrow: hair follicles. Observed on PWD35. g Dot graph of the number of regenerated hair follicles (HF) observed in spiny mouse wounds under different treatments. n = 6 biologically independent animals. Data are presented as mean values ± SEM. * p = 0.0265; ** p = 0.0027; unpaired two-sided t -test. Ctl: control. The images in f represent six out of six experiments performed. The images in e represent 4 out of 4 experiments performed.

Article Snippet: The Twist1 (ab50887), Collagen I (ab34710) and Collagen III (ab7778) antibodies are from Abcam (Cambridge, MA, USA), MMP9 (N2C1, GTX100458) is from GeneTex (Irvine, CA, USA), Snai1 (13099-1-AP), P-cadherin (13773-1-AP), E-cadherin (20874-1-AP) and Zeb2 (14026-1-AP) are from Proteintech (Rosemont, IL, USA).

Techniques: Expressing